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MTOX1303

Sigma-Aldrich

Canine MDR1 Knockout, Human MDR1 Knockin MDCKII Cells

canine kidney (cocker spaniel)

Synonym(s):

MDCKII hMDR1 knockin cells

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

product name

Canine MDR1 Knockout, Human MDR1 Knockin MDCKII Cells,

biological source

canine kidney (cocker spaniel)

Quality Level

200

form

liquid

storage temp.

−196°C

Gene Information

human ... ABCB1(5243)

Application

See technical bulletin for detailed protocols

Features and Benefits

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human MDR1 (P-gp) has been transfected into the MDCKII cell line. Validation studies have shown efflux of standard MDR1 substrates.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines LicenseMDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Deep Agnani et al.
PloS one, 6(10), e25086-e25086 (2011-10-27)
P-glycoprotein, a human multidrug resistance transporter, has been extensively studied due to its importance to human health and disease. In order to understand transport kinetics via P-gp, confluent cell monolayers overexpressing P-gp are widely used. The purpose of this study
G C Hansson et al.
The EMBO journal, 5(3), 483-489 (1986-03-01)
The glycosphingolipids (GSLs) of two sublines of Madin-Darby canine kidney (MDCK) cells, an epithelial cell line, were characterized by t.l.c., antibody overlay and mass spectrometry. The major characteristic which distinguishes the two MDCK cell strains is their trans-epithelial electrical resistance
S Simon et al.
Biochimica et biophysica acta, 1821(9), 1211-1223 (2012-06-14)
Phospholipids are widely used excipients for pharmaceutical formulations, such as for preparing biphasic systems or to solubilize or encapsulate poorly soluble drugs. The present study investigates a new property of this class of substance: its ability to inhibit the efflux

Articles

CRISPR Engineered MDCKII Cells Without Canine P-glycoprotein

Madin-Darby Canine Kidney (MDCK) cells are one of the most widely used cell lines for a variety of research applications, including drug permeability and transporter studies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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