GE28-9909-45
Superdex® 200 Increase 5/150 GL
Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size
Synonym(s):
Size-exclusion chromatography column
About This Item
Recommended Products
material
glass column
polyethylene filter
product line
Superdex®
mol wt
10000-600000 Da (globular proteins)
manufacturer/tradename
Cytiva 28-9909-45
packaging
1 ea of
parameter
<0.75 mL/min flow rate
<0.75 ml/min flow rate
<3.0 Mpa (725 psi)
4-50 μL sample volume
bed volume
~3 mL
column L × I.D.
15 cm × 5 mm
matrix
agarose dextran composite
polymeric
matrix active group
phenyl phase
particle size
8.6 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
Related Categories
General description
Suitability
Storage and Stability
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
This page shows how to perform a purification of His-tagged membrane proteins.
Protocols
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
This page shows how to solubilize membrane proteins with products from Cytiva.
Related Content
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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