GE28-9893-33
HiLoad® 16/600 Superdex® 75 pg
Cytiva 28-9893-33, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size
Synonym(s):
HiLoad 16/600
About This Item
Recommended Products
material
bed support (polyamide)
glass column
glass fiber (reinforced)
mol wt
3000-70000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-33
parameter
<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)
bed volume
120 mL
column L × I.D.
60 cm × 16 mm
matrix
agarose-dextran composite
dextran-agarose particle platform
polymeric
matrix active group
dextran-agarose phase
dextran-agarose phase
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
General description
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Features and Benefits
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
Suitability
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
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Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Protocols
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to refold proteins using gel filtration and products from Cytiva.
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
Related Content
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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