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GE27-4577-01

Sigma-Aldrich

Anti-GST Antibody

Cytiva 27-4577-01

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About This Item

UNSPSC Code:
12352116
NACRES:
NA.32

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

manufacturer/tradename

Cytiva 27-4577-01

packaging

pkg of 0.5 mL, 50 detections

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Anti-GST Antibody is a polyclonal antibody purified from the sera of goats immunized with purified schistosomal GST. Because of its polyclonal nature, it can recognize more than one epitope on GST, thereby improving its capacity for recognizing GST fusion proteins even if some binding sites are masked due to recombinant protein folding.

Application

Western Blots

Features and Benefits

  • For the highly sensitive and specific detection of recombinant schistosomal GST fusion proteins expressed using pGEX vectors
  • Extensively cross-adsorbed to remove antibodies that bind to E. coli proteins and affinity purified using Glutathione Sepharose™
  • Supplied unconjugated for use with any enzyme-conjugated anti-goat antibody
  • Recommended for use in Western blots and dot blots to detect GST fusion proteins

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GST Gene Fusion Vectors
A license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).
Sepharose is a trademark of Cytiva

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Storage Class Code

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Claire E J Moore et al.
Cellular signalling, 28(4), 284-293 (2016-01-23)
Eukaryotic elongation factor 2 kinase (eEF2K) inhibits the elongation stage of protein synthesis by phosphorylating its only known substrate, eEF2. eEF2K is tightly regulated by nutrient-sensitive signalling pathways. For example, it is inhibited by signalling through mammalian target of rapamycin
Apoorva Baluapuri et al.
Molecular cell, 74(4), 674-687 (2019-04-01)
The MYC oncoprotein binds to promoter-proximal regions of virtually all transcribed genes and enhances RNA polymerase II (Pol II) function, but its precise mode of action is poorly understood. Using mass spectrometry of both MYC and Pol II complexes, we

Articles

Troubleshooting Detection Methods for GST-tagged Proteins

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Protocols

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service