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B1140

Sigma-Aldrich

Anti-Human IgG (γ-chain specific)-Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:20,000

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis among many others. Anti-human IgG (γ-chain specific)-biotin antibody can be used in preparation of the double-codified gold nanoparticle label. Goat anti- human IgG (γ-chain specific)-biotin antibody reacts specifically with human IgG but not with other immunoglobulins.
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders
Anti-Human IgG (γ-chain specific)-Biotin antibody is specific for human IgG and does not bind other human immunoglobulins.

Immunogen

Purified human IgG.

Application

Anti-Human IgG (γ-chain specific)-Biotin antibody is suitable for use in ELISA (1:2000) and double-codified gold nanolabel-based immunoanalysis.
Anti-human IgG (γ-chain specific)-biotin antibody can be used in direct ELISA

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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F Lolli et al.
Clinical immunology and immunopathology, 59(2), 314-321 (1991-05-01)
We studied, with sensitive ELISAs, the anti-cardiolipin antibodies of the G, A, and M classes in the cerebrospinal fluid (CSF) and serum of 179 neurological patients. The CSF and serum of 2 systemic lupus erythematosus (SLE) patients presented IgG anti-cardiolipin
Adriano Ambrosi et al.
Analytical chemistry, 79(14), 5232-5240 (2007-06-21)
A novel double-codified nanolabel (DC-AuNP) based on gold nanoparticle (AuNP) modified with anti-human IgG peroxidase (HRP)-conjugated antibody is reported. It represents a simple assay that allows enhanced spectrophotometric and electrochemical detection of antigen human IgG as a model protein. The
S D Litwin et al.
Clinical immunology and immunopathology, 47(1), 75-83 (1988-04-01)
The immunologic characteristics of normal persons with high sera IgD values were analyzed. Elevated sera IgD appeared to be the consequence of increased biosynthesis as reflected by an increased number of IgD-Ig-containing cells, an elevated sera IgD, lambda/kappa ratio, and
Fang Tang et al.
Journal of immunology (Baltimore, Md. : 1950), 186(12), 7264-7268 (2011-05-18)
Six years have passed since the outbreak of severe acute respiratory syndrome (SARS). Previous studies indicated that specific Abs to SARS-related coronavirus (SARS-CoV) waned over time in recovered SARS patients. It is critical to find out whether a potential anamnestic
Lia Vassena et al.
PLoS pathogens, 8(4), e1002636-e1002636 (2012-04-19)
Although treatment with interleukin-7 (IL-7) was shown to transiently expand the naïve and memory T-cell pools in patients with chronic HIV-1 infection receiving antiretroviral therapy (ART), it is uncertain whether a full immunologic reconstitution can be achieved. Moreover, the effects

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