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I4506

Sigma-Aldrich

IgG from human serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym(s):

Human IgG

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About This Item

MDL number:
MFCD00163923
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

unconjugated

Quality Level

300

grade

reagent grade

Assay

≥95% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

composition

Protein, ≥90%

technique(s)

ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable

storage temp.

2-8°C

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General description

Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.

Application

IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia. It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml. IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biotechnology progress, 35(3), e2775-e2775 (2019-01-11)
High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum
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