Skip to Content
Merck
All Photos(1)

Key Documents

A2582

Sigma-Aldrich

Anti-Thioredoxin–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

Synonym(s):

Anti-Thioredoxin

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

Pricing and availability is not currently available.

biological source

rabbit

Quality Level

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

PBS suspension

mol wt

antigen 11.7 kDa

species reactivity

E. coli

analyte chemical class(es)

proteins (thioredoxin)

Looking for similar products? Visit Product Comparison Guide

General description

Thioredoxin is a small electron transport protein. The thioredoxin system consists of thioredoxin, thioredoxin-reductase and NADPH. Thioredoxin obtained from E. coli is made up of a single polypeptide chain of 108 amino acids. It has a molecular weight of 11,700. The protein contains no prosthetic group or bound metals.

Specificity

Specific for natural E. coli and recombinant thioredoxin. It may be used to identify and purify the expression of thioredoxin fusion proteins.

Immunogen

recombinant E. coli thioredoxin.

Application

Anti-Thioredoxin−Agarose antibody produced in rabbit has been used for immunoaffinity purification and immunoprecipitation of thioredoxin from bacterial lysates.
Immunofluorescence was carried out on the cerivcal cancer cell lines SiHa, CaSki, and HeLa using an antibody against the redox proteinThioredoxin.

Biochem/physiol Actions

Thioredoxin is a 11.7kDa electron transport protein that functions as a hydrogen donor during the enzymatic reduction of ribonucleotides to yield deoxyribonucleotides. The thioredoxin system is also involved in the enzymatic reductions of methionine sulfoxide and sulfate. The gene encoding E. coli thioredoxin has been cloned into several plasmids and serves as a fusion partner for the expression of other cloned genes. Hence, the system is useful for the production of soluble fusion proteins in the E. coli cytoplasm. Furthermore, in several instances, these fusion proteins fold correctly and thus display full biological activity.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Prepared using antibody coupled to cyanogen bromide activated agarose at 5 mg antibody per mL resin volume.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Need A Sample COA?

This is a sample Certificate of Analysis (COA) and may not represent a recently manufactured lot of this specific product.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Thioredoxin. 6. The amino acid sequence of the protein from escherichia coli B.
A Holmgren
European journal of biochemistry, 6(4), 475-484 (1968-12-05)
The involvement of the thioredoxin system in the reduction of methionine sulfoxide and sulfate.
P Gonzalez Porqué et al.
The Journal of biological chemistry, 245(9), 2371-2374 (1970-05-10)
Thioredoxin System Regulation in the Central Nervous System: Experimental Models and Clinical Evidence
Silva-AD, et al.
Oxidative Medicine and Cellular Longevity (2014)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service