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Supelco

SUPELCOSIL™ LC-NH₂ (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

EC Number:
276-161-8
UNSPSC Code:
41115700
eCl@ss:
32110501

product name

SUPELCOSIL™ LC-NH2 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L8

Quality Level

200

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

extent of labeling

3% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 5.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amino phase

particle size

5 μm

pore size

120 Å

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).
The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL™ LC-NH2-NP HPLC Columns for additional details).

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
283427-06
283427-05
283427-03
283326-06
283326-04

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Flávia Santana Moraes et al.
Food chemistry, 217, 346-351 (2016-09-25)
An analytical method was developed and validated for the determination of maltodextrin in raw milk, using high-performance liquid chromatography with evaporative light scattering detection. Maltodextrin content was evaluated in adulterated raw milk using a Supelcosil LC-NH2 (25cm×4.6mm) column and isocratic
Maria Veselá et al.
Die Nahrung, 46(4), 251-255 (2002-09-13)
The degradation fo steroid glycoalkaloids (SGAs) has been studied in model solutions. The number of colony forming units (CFU) was determined using a nondirect (cultivation) method during all stages of fermentation. The changes in SGAs content were observed by HPLC
A L Huidobro et al.
Journal of chromatography. A, 1119(1-2), 238-245 (2005-12-21)
Ibuprofen arginate is a rapidly absorbed salt designed to promote more rapid onset of analgesia than commercially available forms of ibuprofen. Ibuprofen and arginine have very different polarities and this becomes in a chromatographic problem, further complicated with the determination

Protocols

HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2

HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2

Chromatograms

application for HPLC

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