ZRB2069
Anti-XLF/NHEJ1 clone 1C16 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Anti-CXCL12/SDF1 Antibody, clone 5I2 ZooMAb® Rabbit Monoclonal, Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C16, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 33.34 kDa
observed mol wt ~38 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... NHEJ1(79840)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1C16 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Non-homologous end-joining factor 1 (XLF/NHEJ1). It targets an epitope within 21 amino acids from the C-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Non-homologous end-joining factor 1 (XLF/NHEJ1).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected XLF/NHEJ1 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected XLF/NHEJ1 in Ramos cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound XLF/NHEJ1 peptide with a KD of 1.1 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 1 µg from a representative lot detected XLF/NHEJ1 in one million Ramos cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected XLF/NHEJ1 in HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected XLF/NHEJ1 in Human prostate tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected XLF/NHEJ1 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected XLF/NHEJ1 in Ramos cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound XLF/NHEJ1 peptide with a KD of 1.1 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 1 µg from a representative lot detected XLF/NHEJ1 in one million Ramos cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected XLF/NHEJ1 in HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected XLF/NHEJ1 in Human prostate tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Non-homologous end-joining factor 1 (UniProt: Q9H9Q4; also known as Protein cernunnos, XRCC4-like factor) is encoded by the NHEJ1 (also known as XLF) gene (Gene ID: 79840) in human. XLF is a ubiquitously expressed DNA repair protein that is involved in non-homologous end joining (NHEJ) and is shown to be essential for double-strand break (DSB) repairs and V(D)J recombination. Together with PAXX, it collaborates with DNA polymerase lambda to promote joining of non-cohesive DNA ends. It may also act in concert with Ku to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. The XRCC4-XLF subcomplex is reported to bind to the DNA fragments of a DSB in a highly diffusive manner and strongly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factor
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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