ZMS1069
Anti-Rhodopsin CT Antibody, clone Rho 1D4 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
Rho 1D4, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 39.01 kDa
observed mol wt ~37 kDa
species reactivity
human
species reactivity (predicted by homology)
mouse, bovine
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone Rho 1D4 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects rhodopsin. It targets an epitope within 9 amino acids from the C-terminal region.
Immunogen
Purified full-length Bovine Rhodopsin.
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human retinal tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Rhodopsin CT in human retina tissue sections.
Tested applications
Western Blotting Analysis: A 1:50,000 dilution from a representative lot detected Rhodopsin CT in Mouse Retina and Human Retina tissue lysates.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Rhodopsin CT in human retina tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rhodopsin CT in ARPE-19 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rhodopsin CT in ARPE-19 cell line.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunohistochemistry (Paraffin) in human retinal tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Rhodopsin CT in human retina tissue sections.
Tested applications
Western Blotting Analysis: A 1:50,000 dilution from a representative lot detected Rhodopsin CT in Mouse Retina and Human Retina tissue lysates.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Rhodopsin CT in human retina tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rhodopsin CT in ARPE-19 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rhodopsin CT in ARPE-19 cell line.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rhodopsin CT, clone Rho 1D4 ZooMAb®, Cat. No. ZMS1069, is a recombinant Mouse monoclonal antibody that detects Rhodopsin and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Rhodopsin (UniProt: P02699; also known as Rho) is encoded by the RHO gene (Gene ID: 509933) in bovine. Rhodopsin is a multi-pass membrane homodimeric protein of the G-protein coupled receptor 1 family that is found in rod shaped photoreceptor cells that mediate vision in dim light. It is synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia. It contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. It is synthesized in the inner segment of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment photosensory cilia. Rhodopsin is required for image-forming vision at low light intensity and is shown to be essential for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling. Mutations in RHO gene in human are known to cause Retinitis pigmentosa 4 that is characterized by retinal pigment deposits and loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors leading to night vision blindness. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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