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Key Documents

T2819

Sigma-Aldrich

Trizma® hydrochloride solution

pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

9.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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General description

Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • to neutralize IgG bound to protein A/G column
  • as a component in BigDye buffer for sequencing reaction
  • to block slides

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Experimental protocol for detecting cyanobacteria in liquid and solid samples with an antibody microarray chip
Blanco Y, et al.
Journal of Visualized Experiments, 142(120), e54994-e54994 (2017)
Using improved technology for filter paper-based blood collection to survey wild Sika deer for antibodies to hepatitis E virus
Yu C, et al.
Journal of Virological Methods, 142(1-2), 143-150 (2007)
Genotypic inference of HIV-1 tropism using population-based sequencing of V3
McGovern RA, et al.
Journal of Visualized Experiments, 142(46), e2531-e2531 (2010)
Tris/Tris (textperiodcentered) HCl: a standard buffer for use in the physiologic pH range
Durst RA and Staples BR
Clinical Chemistry, 18(3), 206-208 (1972)
Hao Wang et al.
Plant physiology, 172(2), 1003-1018 (2016-08-18)
Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway

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