SRP0108

HDAC-6 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonym(s): HD6, Histone deacetylase 6, JM21
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• mol wt: 159 kDa
• packaging: pkg of 50 μg
• NCBI accession no.: BC069243
• UniProt accession no.: Q9UBN7
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... HDAC6(10013)

Description

General description

HDAC6 (histone deacetylase 6) is a member of the HDAC family of enzyme which are generally linked with gene expression and chromatin dynamics. This protein resides exclusively in the cytoplasm and is present in the perinuclear punctate structures as well as the leading edges of the cell.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

HDAC6 (histone deacetylase 6) functions as a microtubule or tubulin deacetylase, which in turn regulates microtubule-dependent chemotactic cell motility. This protein shows colocalization with p150^glued protein, a dynactin component. It contains a ubiquitin binding zinc finger, and interacts with ubiquitinated proteins post-proteasomal inhibition. It is essential for the survival of inflammatory breast cancers (IBCs), though does not function as an oncogene. Treatment of IBC cells with HDAC6 inhibitor ricolinostat (ACY1215) results in the inhibition of IBC cell proliferation both in vitro and in vivo.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3mM DTT, and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable