SBR00069

Rhodamine labeled D-Lysine

Suitable for fluorescent microbial imaging

• Synonym(s): D-Lysine red, FDAA, TDL
• Empirical Formula (Hill Notation): C₃₁H₃₄N₄O₆
• CAS Number: 1417905-39-5
• Molecular Weight: 558.62
• UNSPSC Code: 12352209
• NACRES: NA.21

Properties

• Quality Level: 200
• form: solid
• storage temp.: −20°C

Description

General description

Bacterial Peptidoglycan is a polymer consisting of sugars and amino acids, a mesh-like rigid layer that forms the cell wall. D-Amino Acids (D-AAs) are metabolically incorporated onto the bacterial cell wall by D,D-transpeptidase and/or L,D-transpeptidase. It was recently discovered that those transpeptidases catalyze the metabolic incorporation of exogenous D-AAs with almost no restriction of the side-chain identity. Modification of a D-AA with molecular fluorophores provides fluorescent D-amino acids that can efficiently label in situ peptidoglycan in diverse bacterial species. Rhodamine-Labeled D-lysine (TDL) is a fluorescent derivative of D-lysine that is obtained by a synthetic conjugation of D-lysine to the fluorophore. Rhodamine Labeled D-lysine has high biocompatibility and suitability for labeling peptidoglycans in live bacteria. Additionally, it can be used in tandem with other stains such as FITC-Labeled D-Alanine (SBR00049) to distinguish between different bacteria. Other compatible products useful for live staining include: Rhodamine B Labeled Polymyxin B, FITC Labeled D-Lysine, FITC Labeled Vancomycin and Dansyl Labeled Polymyxin B (SBR00036, SBR00050, SBR00028, SBR00029 and SBR00047).

Application

Fluorescent labeled D-AAs (FDAAs) can be used for many applications including:

• Bacterial cell wall morphology
• Bacterial cell wall formation or remodeling activity
• Bacterial viability/activity
• Identify bacterial activity on surfaces or in substances
• Differentiation between various bacterial strains according to their incorporation profile of different D amino acids and sugars

Analysis Note

• Fluorescent microscopy application: Rhodamine Labeled D-lysine has excitation/emission wavelength range at 550/570 nm.
• The recommended working concentration in fluorescent microscopy imaging application is between 250 µM-500 µM in working medium.
• Aliquots of the DMSO solution can be stored at -20 °C, protected from light for at least one month.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable