SAB4200784
Anti-Cellular Fibronectin antibody, Mouse monoclonal
clone FN-3E2, hybridoma cell culture supernatant
Synonym(s):
Anti-CIG, Anti-Cold-insoluble globulin, Anti-FN
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All Photos(2)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
FN-3E2, monoclonal
form
buffered aqueous solution
mol wt
~220-260 kDa
species reactivity
chicken, rat, human, mouse
technique(s)
immunoblotting: suitable
immunofluorescence: 1:2,000-1:4,000 using human foreskin fibroblast Hs68 cells
immunohistochemistry: suitable
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FN1(2335)
General description
Anti-Cellular Fibronectin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the FN-3E2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the antigens released in culture from a breast cancer cell line. Fibronectin (FN), also known as cold-insoluble globulin (CIG), is an extracellular matrix multi-domain glycoprotein composed of two nearly identical disulphide bound polypeptides. Fibronectin 1 is a glycoprotein, that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Cellular fibronectin is produced by various cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells, and myocytes.
Immunogen
Antigens released in culture from a breast cancer cell line
Application
Anti-Cellular Fibronectin antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Anti-cellular Fibronectin antibody, mouse monocloneal has been used in various immunochemical techniques including immunoblotting (220-260 kDa), immunofluorescence, and immunohistochemistry.
Biochem/physiol Actions
Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, haemostasis and thrombosis. It is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism.
Physical form
The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains fetal calf serum.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The extracellular matrix proteins laminin and fibronectin contain binding domains for human plasminogen and tissue plasminogen activator.
Moser TL, et al.
The Journal of Biological Chemistry, 268(25), 18917-18923 (1993)
Receptors for cold-insoluble globulin (plasma fibronectin) on human monocytes.
Bevilacqua MP, et al.
The Journal of Experimental Medicine, 153(1), 42-60 (1981)
E L George et al.
Development (Cambridge, England), 119(4), 1079-1091 (1993-12-01)
To examine the role of fibronectin in vivo, we have generated mice in which the fibronectin gene is inactivated. Heterozygotes have one half normal levels of plasma fibronectin, yet appear normal. When homozygous, the mutant allele causes early embryonic lethality
R Manabe et al.
The Journal of cell biology, 139(1), 295-307 (1997-10-06)
Fibronectin (FN) has a complex pattern of alternative splicing at the mRNA level. One of the alternatively spliced segments, EDA, is prominently expressed during biological processes involving substantial cell migration and proliferation, such as embryonic development, malignant transformation, and wound
Yong Mao et al.
Matrix biology : journal of the International Society for Matrix Biology, 24(6), 389-399 (2005-08-03)
The extracellular matrix provides a framework for cell adhesion, supports cell movement, and serves to compartmentalize tissues into functional units. Fibronectin is a core component of many extracellular matrices where it regulates a variety of cell activities through direct interactions
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