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N1408

Sigma-Aldrich

Nerve Growth Factor β Human

>97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Synonym(s):

HSAN5, NGFB, hNGFβ

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About This Item

MDL number:
MFCD00164982
UNSPSC Code:
12352202
NACRES:
NA.77

product name

NGF-β human, from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

biological source

human

Quality Level

200

recombinant

expressed in NSO cells

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.15-3.0 ng/mL ED50/EC50

quality

endotoxin tested

packaging

pkg of 5X0.1 mg
pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

solubility

water: soluble 0.1 g/L at 20 °C

UniProt accession no.

P01138

storage temp.

−20°C

Gene Information

human ... NGF(4803)

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General description

The nerve growth factor β (NGFB) gene is mapped to human chromosome 1p13.2.

Application

NGF-β human has been used:
  • in protein pin arrays
  • to induce U87-MG (glioblastoma cell line) cell proliferation
  • in drug release kinetics

Biochem/physiol Actions

Nerve growth factor β (NGFB) acts as a key mediator in neuronal and non-neuronal development, differentiation, and survival. Polymorphism of NGFB gene leads to hereditary sensory and autonomic neuropathies. Charcot arthropathy and thermal insensitivity are few of the related symptoms. NGFβ is known to promote cancer cell proliferation and metastasis, also mediates angiogenesis. It facilitates the development of various cancer including, oral, breast, lungs, pancreatic and prostate cancers. Elevated expression of NGFβ contributes to progression of human cholangiocarcinoma. In animal models, NGFβ is observed to prevent cholinergic neuron loss and enhances memory. NGF-β promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

Physical form

Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin.

Analysis Note

The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K A Crutcher et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 13(6), 2540-2550 (1993-06-01)
A two-site ELISA and a bioassay were used to detect NGF-like activity in human brain tissue. Both assays detected mouse NGF and recombinant human NGF with approximately equal sensitivity, whereas the antibodies showed little cross-reactivity with the recombinant human proteins
Nerve Growth Factor Stimulates Glioblastoma Proliferation through Notch1 Receptor Signaling
Park JC, et al.
Journal of Korean Neurosurgical Society, 61(4), 441-441 (2018)
Mahmoud Aarabi et al.
Fertility and sterility, 102(2), 440-447 (2014-06-09)
To determine the levels of postacrosomal WW binding protein (PAWP) in the spermatozoa of men that were used clinically for intracytoplasmic sperm injection (ICSI) and to correlate them with infertility treatment outcomes. Prospective clinical and laboratory study. University-based laboratory and
S Korsching
The Journal of neuroscience : the official journal of the Society for Neuroscience, 13(7), 2739-2748 (1993-07-01)
The neurotrophic factor concept in its basic form envisages that innervated tissues produce a signal for the innervating neurons for the selective limitation of neuronal death occurring during development (Purves, 1986; Oppenheim, 1991). This concept arose several decades ago on
A phase 1 clinical trial of nerve growth factor gene therapy for Alzheimer disease
Tuszynski MH, et al.
Nature Medicine, 11(5), 551-551 (2005)
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