MAK542

Glucose Uptake Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC Code: 41116104

Properties

• detection method: colorimetric
• relevant disease(s): endocrinological disorders, diabetes; cancer
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Glucose transport systems are responsible for transporting glucose across cell membranes. Measuring uptake of 2-deoxyglucose (2-DG), a glucose analog, in tissues and cells is widely accepted as a reliable method to estimate the amount of glucose uptake and to investigate the regulation of glucose metabolism and mechanism of insulin resistance. ​

The Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay in tissues or cultured cells. In this assay, 2-DG is taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). The non-metabolizable 2-DG6P accumulates in the cells and is proportional to glucose uptake by cells. The accumulated 2-DG6P is enzymatically oxidized and generates NADPH, which is specifically monitored by a chromogenic NADPH sensor. The signal can be read by a spectrophotometric plate reader by reading the OD ratio at wavelength 570 nm to 610 nm.

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290 - H315 - H319 - H412
• Precautionary Statements: P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3