MAK190
ATP Assay Kit
Sufficient for 100 Colorimetric or Fluorometric tests
Synonym(s):
Adenosine triphosphate Assay Kit
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Recommended Products
detection method
colorimetric
fluorometric
relevant disease(s)
cancer; neurological disorders
storage temp.
−20°C
General description
Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes. Genetic disorders such as Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa affect the generation of ATP in the mitochondria.
Application
ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.
Suitability
Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.
Principle
The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.
Other Notes
The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.
Kit Components Only
Product No.
Description
- ATP Assay Buffer
- ATP Probe, in DMSO
- ATP Converter
- Developer Mix
- ATP Standard, 1.0 μmole
replaced by
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Cost of surviving sepsis: A novel model of recovery from sepsis in Drosophila melanogaster.
Kaynar A M, et al.
Intensive care medicine experimental, 4(1), 4-4 (2016)
Rosalba Carrozzo et al.
Bioscience reports, 27(1-3), 105-112 (2007-05-09)
Mitochondrial disorders encompass any medical specialty and affect patients at any age. Likewise, the spectrum of clinical and genetic signatures of these disorders is ample, making a precise diagnosis difficult. We will report some of the major clinical phenotypes observed
Sandra Aurora Niño et al.
ACS chemical neuroscience, 10(1), 323-336 (2018-08-25)
Worldwide, every year there is an increase in the number of people exposed to inorganic arsenic (iAs) via drinking water. Human populations present impaired cognitive function as a result of prenatal and childhood iAs exposure, while studies in animal models
Melatonin Balance the Autophagy and Apoptosis by Regulating UCP2 in the LPS-Induced Cardiomyopathy.
Pan P, et al.
Molecules (Basel), 23(3), 675-675 (2018)
E Alirol et al.
Oncogene, 25(34), 4706-4716 (2006-08-08)
Mitochondria are key players in several cellular functions including growth, division, energy metabolism, and apoptosis. The mitochondrial network undergoes constant remodelling and these morphological changes are of direct relevance for the role of this organelle in cell physiology. Mitochondrial dysfunction
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service