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MAK066

Sigma-Aldrich

Lactate Dehydrogenase Activity Assay Kit

sufficient for 500 colorimetric tests

Synonym(s):

LDH Activity Assay Kit, LDH Assay, Lactic Dehydrogenase Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 500 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder; cancer

storage temp.

−20°C

Gene Information

human ... LDHA(3939) , LDHB(3945)
mouse ... LDHA(16828) , LDHB(16832)
rat ... LDHA(24533) , LDHB(24534)

General description

The new Lactate Dehydrogenase Assay Kit, MAK464, is now available! Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. LDH is also elevated in certain pathological conditions such as cancer. Quantification of LDH has a broad range of applications.




Application

Lactate Dehydrogenase Activity Assay Kit has been used for measuring the activity of lactate dehydrogenase (LDH) in the cell culture medium.
Lactate Dehydrogenase Activity Assay Kit has been used to determine the concentration of lactate dehydrogenase in samples.

Principle

In this kit, LDH reduces NAD to NADH, which is specifically detected by colorimetric (450 nm) assay. The LDH Activity Assay kit quantifies LDH activity in variety of biological samples. The assay is quick, convenient, and sensitive.

replaced by

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

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Articles

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We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

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