L6544

LuminoCt^® SYBR^® Green qPCR ReadyMix^™

For fast SYBR Green quantitative PCR

• Synonym(s): LuminoCt^®
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 100 reactions; sufficient for 2000 reactions; sufficient for 500 reactions
• feature: dNTPs included; hotstart
• technique(s): qPCR: suitable
• color: colorless
• input: purified DNA
• application(s): agriculture
• compatibility: Bio-Rad MyiQ; Bio-Rad iCycler iQ; Bio-Rad iQ 5; for use with ABI 5700; for use with ABI 7000; for use with ABI 7300; for use with ABI 7500 Fast; for use with ABI 7500; for use with ABI 7700; for use with ABI 7900 Fast; for use with ABI 7900 HT; for use with ABI 7900; for use with ABI StepOne; for use with ABI StepOnePlus; for use with ABI ViiA 7; for use with Bio-Rad CFX384; for use with Bio-Rad CFX96; for use with Bio-Rad MJ Chromo4; for use with Bio-Rad MJ Opticon 2; for use with Bio-Rad MJ Opticon Cepheid SmartCycler; for use with Bio-Rad MJ Opticon; for use with Bio-Rad MiniOpticon; for use with Eppendorf^® Mastercycler ep realplex2 s; for use with Eppendorf^® Mastercycler ep realplex; for use with Illumina Eco qPCR; for use with Qiagen Corbett Rotor-Gene 3000; for use with Qiagen Corbett Rotor-Gene 6000; for use with Qiagen Corbett Rotor-Gene Q; for use with Roche LightCycler 480; for use with Strategene Mx3000P; for use with Strategene Mx3005P; for use with Strategene Mx4000
• detection method: SYBR^® Green
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

LuminoCt^® SYBR^® Green qPCR ReadyMix is ready to use master mix that combines the performance enhancements of our JumpStart^™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart^™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

• Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
• ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
• Kit is not compatible with qPCR instruments that utilize glass capillary tubes

Application

LuminoCt^® SYBR^® Green qPCR ReadyMix^™ has been used for fast quantitative polymerase chain reaction (qPCR) and quantitative RT-PCR amplifications. It has also been used in qPCR for measuring gene expression.

Features and Benefits

• Ideal for performing high-throughput, quantitative PCR applications
• The master mix allows consistency from one reaction to the next
• Reduced preparation time and reduced risk of contamination from multiple pipetting steps
• The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
• LuminoCt^® ReadyMixes require the use of small amplicons (<200bp) for optimal results
• This master mix is compatible with commercial primer sets, including TaqMan^® assays

• Assay results in as little as 25 minutes
• Unsurpassed accuracy, precision, and sensitivity
• Virtually eliminates the need to optimize assay parameters

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Other Notes

• At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
• Sigma recommends designing primers and probes with Beacon Designer software
• To order primers and probes from Sigma, visit: www.sigma.com/oligos

Legal Information

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

SabreLite is a registered trademark of Pelican Products, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Only

• 2 X

Kit Components Also Available Separately

• R4526 100X ROX internal reference dye

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1