L0770
Lectin from Pisum sativum (pea)
FITC conjugate, lyophilized powder
Synonym(s):
Pisum sativum agglutinin, PSA
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About This Item
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conjugate
FITC conjugate
Quality Level
form
lyophilized powder
potency
<20 μg per mL agglutination activity
composition
Protein, ≥95% biuret
extent of labeling
2-4 mol FITC per mol protein (Lowry).
impurities
salt, free
storage temp.
−20°C
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General description
Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α-D-glucose and α-D-mannose.
Application
Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns
Biochem/physiol Actions
Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).
PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.
Preparation Note
Conjugates are prepared from affinity purified lectin.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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