K4377
Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse
clone KP-53, ascites fluid
Synonym(s):
Monoclonal Anti-Human Kappa Light Chain (Bound and Free)
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
KP-53, monoclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:10,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.
Specificity
The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.
Application
Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:
- immunodetection
- in western blot analysis
- in intracellular kappa light chain staining for flow cytometric analysis
Physical form
Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse is provided as ascites fluid with 0.1% sodium azide (see MSDS) as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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