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IAK0108

Sigma-Aldrich

Phagocytosis Assay Kit

Green Zymosan, sufficient for 100 fluorometric assays

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About This Item

UNSPSC Code:
12352207

suitability

suitable for fluorescence

storage temp.

2-8°C

General description

Phagocytosis is a cellular process for ingesting and eliminating microorganisms, foreign substances, and apoptotic cells. It is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae) and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.

Application

The Phagocytosis Assay Kit (Green Zymosan) uses fluorescently pre-labeled Zymosan particles to detect and quantify in vitro phagocytosis by fluorescent microscope, spectrophotometer (Ex/Em = 490/520 nm) or flow cytometry.

Suitability

This kit is suitable for screening activators and/or inhibitors of phagocytosis and Toll-like receptor ligands (TLR) in mammalian cells.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3


Certificates of Analysis (COA)

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