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GERPN5785

Vistra ECF™ Substrate

Cytiva RPN5785

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About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN5785

color

ECF substrate: Off-white/white to yellowish ECF substrate dilution buffer: Colorless

shipped in

wet ice

storage temp.

2-8°C

General description

Alkaline phosphatase dephosphorylates ECF™ Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer.

Application

Vistra ECF™ substrate has been used in Western blotting.

Features and Benefits

  • A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
  • For use in western blotting, dot, and slot blotting applications
  • Sensitivity comparable to chemiluminescence
  • The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
  • Eliminates need for darkroom and film

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECF is a trademark of Cytiva

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H318,H315,H373

Storage Class Code

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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