GALS
β-Galactosidase Reporter Gene Staining Kit
Synonym(s):
β-galactosidase, LacZ
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.32
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usage
kit sufficient for 100 tests (using a 3.5 cm dish)
Quality Level
technique(s)
microbe id | staining: suitable
shipped in
dry ice
storage temp.
−20°C
General description
β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
Application
β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:
- sagittal brain sections in histoenzymology
- pancreas or colon sections in β-galactosidase reporter gene assay
- mice E10.5 -11.5 embryo and embryo sections
Other Notes
The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Supplementary Hazards
Storage Class Code
3 - Flammable liquids
Flash Point(F)
132.8 °F
Flash Point(C)
56 °C
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