GALS

β-Galactosidase Reporter Gene Staining Kit

• Synonym(s): β-galactosidase, LacZ
• UNSPSC Code: 12161503
• NACRES: NA.32

Properties

• usage: kit sufficient for 100 tests (using a 3.5 cm dish)
• Quality Level: 200
• technique(s): microbe id | staining: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Application

β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:

• sagittal brain sections in histoenzymology
• pancreas or colon sections in β-galactosidase reporter gene assay
• mice E10.5 -11.5 embryo and embryo sections

Other Notes

The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

Safety Information

• Pictograms: GHS02,GHS06,GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H226,H302 + H312,H314,H317,H331,H334,H335,H341,H350,H412
• Precautionary Statements: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Supplementary Hazards: EUH032
• Storage Class Code: 3 - Flammable liquids
• Flash Point(F): 132.8 °F
• Flash Point(C): 56 °C