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F6257

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Sheep Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

sheep

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:32

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule)-FITC antibody is specific for all mouse IgG subclasses. Affinity isolated antigen specific antibody is obtained from sheep anti-mouse IgG antiserum by immunospecific purification. The antibody preparation is then conjugated to Sigma Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a working antibody dilution of 1:32. For immunofluorescence of human ovarian adenocarcinoma cells stained with anti-integrin primary antibodies a working dilution of 1:50 was used. Immunolocalization of human spermatids was done using antibody dilution of 1:100. This antibody was also used for immunofluorescent tagging of leaf cells of Arabidopsis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sylvie Maubant et al.
International journal of cancer, 97(2), 186-194 (2002-01-05)
In order to elucidate the mechanisms underlying the development of chemoresistance in ovarian cancer, we have previously established the IGROV1-R10 cisplatin-resistant cell line by mimicking a clinical protocol of drug administration on IGROV1 human ovarian carcinoma cells. Both IGROV1 and
U I Ezeh et al.
Human reproduction (Oxford, England), 13(11), 3061-3065 (1998-12-16)
Limiting testicular biopsy for intracytoplasmic sperm injection (ICSI) to those with a high chance of having testicular spermatozoa has not been possible because of the poor predictive value of current clinical and laboratory methods. In order to predict testicular pathology
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Sophie Bouton et al.
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Robert R Gilmont et al.
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Muscle replacement for patients suffering from extensive tissue loss or dysfunction is a major objective of regenerative medicine. To achieve functional status, bioengineered muscle replacement constructs require innervation. Here we describe a method to bioengineer functionally innervated gut smooth muscle

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