ATHOS

93122325, Porcine thyroid, Epithelial

• UNSPSC Code: 41106514
• biological source: Porcine thyroid
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Thyroglobulin, plasminogen activator (u-PA)
• receptors: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone

Properties

• Product Name: ATHOS, 93122325
• biological source: Porcine thyroid
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Thyroglobulin, plasminogen activator (u-PA)
• receptors: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Porcine thyroid epithelial

Cell Line Description

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.

Application

Mechanism of thyroid follicle reconstruction

Culture Medium

Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Other Notes

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