MCA-RH 7777

90021504, rat liver, Hepatocyte

• UNSPSC Code: 41106514

Properties

• Product Name: MCA-RH 7777, 90021504
• biological source: rat liver
• growth mode: Adherent
• karyotype: Not specified
• morphology: Hepatocyte
• products: Alpha-foetoprotein
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat Buffalo hepatoma

Cell Line Description

Derived from the Morris hepatoma 7777 (obtained from a male Buffalo strain rat). The original tumour was induced with N-2 fluorenylphthalamic acid. Particularly interesting for oncogeny studies since the cells produce α-fetoprotein (AFP).

Application

Tumourigenicity and oncogeny studies

Culture Medium

Swimm′s S-77 + 4mM Glutamine + 20% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Split semi-confluent cultures 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. NB: Cells are shed from the monolayer before confluency is achieved.

Other Notes

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