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product name
KNRK, 87061001
biological source
rat kidney
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Growth factors
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Normal rat kidney, Kirsten MSV transformed
Cell Line Description
Derived from normal rat kidney transformed by Kirsten sarcoma virus. Cells do not produce virus but carry the virus genome. In serum free medium the cell line produces growth factors capable of stimulating quiescent cells to grow in monolayers or in soft agar.
Application
Transformation, growth factors and virus studies: Kirsten MSV
Culture Medium
DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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