Rolf B1.T

3071601, rat nose, Epithelial-like

• UNSPSC Code: 41106514

Properties

• Product Name: Rolf B1.T, 03071601
• biological source: rat nose
• growth mode: Adherent
• karyotype: Normal rat chromosome complement
• morphology: Epithelial-like
• products: cadherin, tenascin, laminin, N-CAM, GFAP, p75 NTR
• receptors: p75 NTR
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Adult rat olfactory nerve ensheathing cells

Cell Line Description

Rolf B1.T cells arose from a spontaneous immortalisation event - retaining characteristics of normal cells. The cells are dependent on serum growth factors for growth. They constitutively express glial fibrillary acidic protein, S100, the low affinity neurotrophin receptor p75 NTR, laminin, tenascin, and NCAM. These cells support the regrowth of neurites from adult rat retinal ganglion cells. They have potential value in research into mechanisms of glial support for axonal regeneration from adult mammalian central neurons and other aspects of differentiated glial cell biology. Epithelial-like or fusiform (inducible).

Application

Neural research including axonal regeneration.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 - 1:20 e.g. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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