C5867

Anti-Ciliated Cell Marker antibody, Mouse monoclonal

clone LhS 28, purified from hybridoma cell culture

• MDL number: MFCD08705577
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: LhS 28, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~45 kDa
• species reactivity: human, hamster
• concentration: ~2 mg/mL
• technique(s): electron microscopy: suitable; immunocytochemistry: 1-2 μg/mL using BHK a21 cells; immunohistochemistry: suitable; microarray: suitable; western blot: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Ciliated Cell Marker antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma LhS 28 produced by the fusion of mouse myeloma cells (Sp2/0 cells) and splenocytes from BALB/c mice.

Specificity

Anti-Ciliated Cell Marker antibody, Mouse monoclonal recognizes an antigen in human and hamster ciliated cells.

Immunogen

BHK a21 cytoskeletal preparation.

Application

Anti-Ciliated Cell Marker antibody, Mouse monoclonal may be used in:

• immunoelectron microscopy
• immunoblotting
• immunohistochemistry
• immunocytochemistry

May be used in the detection of ciliated cells and for investigating mechanisms of ciliogenesis.

Biochem/physiol Actions

The mouse hybridoma LhS 28 secretes a monoclonal antibody that recognizes the antigen 44-45 kDa protein associated with the ciliary basal bodies. The antigen recognized by LhS 28 is present in the 9+0 microtubule arrays in the basal body structure of the cilium. It serves as an unequivocal marker for in situ detection and in vitro studies of human ciliated epithelial cells. LhS28 is a potential marker for tubal metaplasia identification and may be useful in recognizing low grade cervical glandular intraepithelial neoplasia. It is effective in detecting ciliogenesis and ciliated cells associated with normal and pathological human specimens

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable