B9673

1-Bromo-3-chloropropane

for isolation of RNA

• Synonym(s): 1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
• Linear Formula: Cl(CH₂)₃Br
• CAS Number: 109-70-6
• Molecular Weight: 157.44
• Beilstein: 605278
• EC Number: 203-697-1
• MDL number: MFCD00000998
• UNSPSC Code: 41105515
• PubChem Substance ID: 24892149
• NACRES: NA.52

Properties

• grade: for molecular biology
• Assay: ≥98.5% (GC)
• form: liquid
• refractive index: n20/D 1.486 (lit.)
• bp: 144-145 °C (lit.)
• density: 1.592 g/mL at 25 °C (lit.)
• storage temp.: room temp
• SMILES string: ClCCCBr
• InChI: 1S/C3H6BrCl/c4-2-1-3-5/h1-3H2
• InChI key: MFESCIUQSIBMSM-UHFFFAOYSA-N

Description

General description

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Application

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:

• feline teeth
• mice brain and liver tissues
• human ectocervical epithelial cells
• placental samples
• human mesenchymal stromal cells (hMSCs)

Features and Benefits

• less toxic alternative to chloroform
• compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Other Notes

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302,H331,H335,H341,H412
• Precautionary Statements: P202 - P261 - P273 - P301 + P312 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves