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53557

Sigma-Aldrich

FITC–DEAE–Dextran

average mol wt 3,000-6,000

Synonym(s):

FITC–DEAE–Dextran 4, Fluorescein isothiocyanate–Diethylaminoethyl–Dextran

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About This Item

UNSPSC Code:
12352116
NACRES:
NA.32

form

solid

Quality Level

mol wt

average mol wt 3,000-6,000

composition

nitrogen content, 2-5%

extent of labeling

0.001-0.020 mol/mol FITC glucose

fluorescence

λex 493 nm; λem 518 nm in 0.1 M Tris pH 8.0

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Application

FITC-DEAE-Dextran 4 is a cationic fluorescent dextran that may be used to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-DEAE-Dextran 4 may be use to compare selectivity versus neutral FITC-Dextran 4 or anionic FITC-CM-Dextran 4.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J K Leypoldt et al.
Kidney international, 43(4), 837-844 (1993-04-01)
The influence of molecular charge on macromolecule transport during peritoneal dialysis was assessed by determining transperitoneal transport rates for fluorescent-labeled macromolecules (molecular radii from 15 to 40 A) that differed only in molecular charge: neutral dextran, anionic dextran sulfate and
Reduced diffusion of charge-modified, conformationally intact anionic Ficoll relative to neutral Ficoll across the rat glomerular filtration barrier in vivo.
Axelsson J, Sverrisson K, Rippe A, et al.
American Journal of Physiology: Renal Physiology, 301, F708-F712 (2011)
Carrier and dose effects on the pharmacokinetics of T-0128, a camptothecin analogue-carboxymethyl dextran conjugate, in non-tumor- and tumor-bearing rats.
Harada M, Murata J, Sakamura Y, et al.
J. Controlled Release, 71, 71-86 (2001)

Articles

Additional information about FITC-labelled polysaccarides including FITC-Dextran, FITC-DEAE-dextran (FDD), FITC-carboxymethyl-dextran (FCM-Dextran). This page discusses the impact of weight-average molecular weight on the respective molecule dimensions and other physical properties

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