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C0537

Sigma-Aldrich

5-Carboxyfluorescein

≥99% purity (HPLC), powder

Synonym(s):

5-FAM

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About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
76823-03-5
Molecular Weight:
376.32
Beilstein:
57037
MDL number:
MFCD00036874
UNSPSC Code:
12171500
PubChem Substance ID:
24892258
NACRES:
NA.47

product name

5-Carboxyfluorescein, ≥99% (HPLC)

Quality Level

200

Assay

≥99% (HPLC)

form

powder

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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Application

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
088M4144V
116M4127V
13161101
13161101
066M4115V

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Relation between insulin resistance and fast-migrating LDL subfraction as characterized by capillary isotachophoresis.
Zhang B, et al.
Journal of Lipid Research, 46, 2265-2265 (2005)
M Goetz et al.
Endoscopy, 39(4), 350-356 (2007-04-12)
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Sabina Gerber et al.
The Journal of biological chemistry, 288(13), 8849-8861 (2013-02-06)
N-Linked glycosylation is an essential post-translational protein modification in the eukaryotic cell. The initial transfer of an oligosaccharide from a lipid carrier onto asparagine residues within a consensus sequon is catalyzed by oligosaccharyltransferase (OST). The first X-ray structure of a
Kotyk A and Slavik J
Intracellular pH and its Measurement (1989)
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