Skip to Content
Merck
All Photos(1)

Key Documents

More Documents

25668

Sigma-Aldrich

Chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Synonym(s):

Chloroform:Isoamyl Alcohol Solvent Mix

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00171265
UNSPSC Code:
12352401
PubChem Substance ID:
329752812
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioUltra

Assay

≥99.5% (chloroform + isoamyl alcohol, GC)

form

liquid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.001% free alkali (as NH3)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤0.5 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.1
λ: 280 nm Amax: 0.07

SMILES string

ClC(Cl)Cl.CC(C)CCO

InChI

1S/C5H12O.CHCl3/c1-5(2)3-4-6;2-1(3)4/h5-6H,3-4H2,1-2H3;1H

InChI key

BKHZIBWEHPHYAI-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions.

Components

mixture of chloroform and isoamyl alcohol in the ratio of 49:1 (v/v)

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Central nervous system, Liver,Kidney

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCF6185
BCCC4649
BCBZ3927
BCBW7561
BCBS6064V

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Bacterial RNA isolation.
Ares M.
Cold Spring Harbor Protocols, 1012, 1024-1027 (2012)
Purification of RNA by SDS solubilization and phenol extraction.
Rio DC, Ares M Jr, Hannon GJ, Nilsen TW.
Cold Spring Harbor Protocols, 2010, 5439-5439 (2010)
D.M. Wallace
Methods in Enzymology, 152, 35-35 (1987)
Marion Poirier et al.
ChemMedChem, 14(2), 224-236 (2018-12-07)
By screening a focused library of kinase inhibitor analogues in a phenotypic co-culture assay for angiogenesis inhibition, we identified an aminotriazine that acts as a cytostatic nanomolar inhibitor. However, this aminotriazine was found to be completely inactive in a whole-kinome
Lu Zhao et al.
Phytochemical analysis : PCA, 23(6), 647-650 (2012-05-04)
Cotton is an important economic crop. Genetic, developmental and molecular studies of cotton require high-quality total RNA from different tissues. Due to the richness in polyphenols and polysaccharides, the Trizol-based methods and other commercial kits are unsuitable for RNA isolation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service