10102121001

Adenosine Deaminase (ADA)

from calf intestine

• Synonym(s): ADA, Adenosine Deaminase
• Enzyme Commission number: 3.5.4.4 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bovine intestine
• Quality Level: 100
• form: suspension
• specific activity: ~200 units/mg protein (At 25 °C with adenosine as the substrate.)
• packaging: pkg of 1 mL (10 mg)
• manufacturer/tradename: Roche
• technique(s): activity assay: suitable
• color: white
• optimum pH: 7.0-7.4
• solubility: water: miscible
• suitability: suitable for chemiluminescence
• NCBI accession no.: AAF91430
• UniProt accession no.: P56658
• application(s): life science and biopharma
• foreign activity: AMP-deaminase <0.01%; AP <0.01% (with pre incubation); Guanase <0.01%; NP <0.01%
• storage temp.: 2-8°C
• Gene Information: bovine ... ADA(280712)

Description

General description

Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Chemiluminescent adenosine assay.

Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.

Biochem/physiol Actions

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Quality

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available