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Y04C-02-5PK

Millipore

CellASIC ONIX plate for haploid yeast cells (4 chamber, 3.5-5 micron)

The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

material

PMMA
glass
polycarbonate
polydimethylsiloxane (PDMS)

manufacturer/tradename

CellASIC® ONIX

W × L × H

85.48 mm × 127.76 mm × 14.35 mm

compatibility

for use with cells sized 3.5–5.0 μm

shipped in

ambient

Related Categories

General description

The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining the x,y position over time.

Application

CellASIC® ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) has been used in live-cell microscopy and analysis of Schizosaccharomyces pombe cells. It has also been used in live cell imagining of Saccharomyces cerevisiae strains. This CellASIC ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) is also suitable for:
  • Time-lapse analysis of yeast cells
  • Long-term continuous perfusion experiments
  • Solution exchange experiments (induction, inhibition, drug dosing, etc.)
  • Comparison of up to 4 different cell types or exposure conditions(media components) in parallel
  • Cell division tracking (follow mother/daughter cells over generations)
  • Temperature and gas atmospheric control (temperature shift, anoxic conditions etc.)

Features and Benefits

  • Compatible with CellASIC® ONIX2 Microfluidic system and ONIX2 Manifolds
  • Enables perfusion-based, long-term, live-cell analysis with solutionswitching
  • The plate offers a controlled and dynamicmicroenvironment for cells

Legal Information

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Ping Wei et al.
Nature, 488(7411), 384-388 (2012-07-24)
Bacterial pathogens have evolved specific effector proteins that, by interfacing with host kinase signalling pathways, provide a mechanism to evade immune responses during infection. Although these effectors contribute to pathogen virulence, we realized that they might also serve as valuable
A Mammalian-like DNA damage response of fission yeast to nucleoside analogs.
Sabatinos SA, Mastro TL, Green MD, Forsburg SL
Genetics, 193 (1), 143-157 (2013)
Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast.
Clara Bermejo, Farzad Haerizadeh, Hitomi Takanaga, Diane Chermak, Wolf Frommer
Nature Protocols, 6, 1806-1817 (2011)
Commitment to a cellular transition precedes genome-wide trasncriptional change.
Doncic A, Falleur-Fettig M, Skotheim J
Molecular Cell, Vol. 43, Issue 4, 515-527 (2011)
Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.
Sabatinos SA, Green MD, Forsburg SL
Molecular and cellular biology, 32 (24), 4986-4997 (2012)
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Articles

Demonstration of long-term culture and in-plate staining protocols using the CellASIC® ONIX Microfluidic platform

This page shows the long-term culture and in-plate staining protocols using the CellASIC ONIX Microfluidic platform.

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