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TGFBMAG-64K-03

Millipore

MILLIPLEX® TGFß Magnetic Bead 3 Plex Kit - Immunology Multiplex Assay

for quantification of TGFß1, TGFß2 and TGFß3 in multiple species

Synonym(s):

3 Plex TGFß Assay, TGFß Magnetic Bead Kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

horse, human, nonhuman primates, feline, pig, mouse, rat, canine

manufacturer/tradename

Milliplex®

assay range

accuracy: 62-128%
sensitivity: 8-12 pg/mL
standard curve range: 9.8-2,500 pg/mL

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

The transforming growth factor beta (TGFβ) system, a superfamily of cytokines as well as signaling pathways, is highly conserved throughout the animal kingdom. TGFβ functions in immune cell development, angiogenesis, wound healing and embryonic development, bone development and plays a critical role in immune response, heart disease, and cancer. In its normal state TGFβ is one of the few classes of proteins able to inhibit cell growth by halting mitosis at the G1 state, inducing cell differentiation or apoptosis. However, during oncogenesis mutations in the TGFβ signaling pathway result in tumor cell resistance to the effects of normally functioning TGFβ, causing proliferation without regulation. Initial research suggests that VE-cadherin may enhance the mutated TGFβ signaling pathway.

The MILLIPLEX® TGFβ 1, 2 and 3 Panel is a 3-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma and cell/tissue culture samples: TGFβ1, TGFβ2, TGFβ3. The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: TGF-β1, TGF-β2, TGF-β3
  • Recommended Species: In addition to human, TGF-β1, TGF-β2, TGF-β3 can be detected in the serum of the following species: mouse, rat, mini pig, hamster, guinea pig, rhesus monkey and cynomolgus monkey samples. Additionally, TGF-β1 and TGF-β2 can be detected in the serum of horse, rabbit and canine. However, the exact amount of cross reactivity has not been determined.
  • NOTE: This assay requires a sample acidification treatment using the provided reagents and protocol.
  • Recommended Sample Types: Serum, plasma, cell/tissue culture supernatants and lysates
  • Recommended Sample Dilution: 25 μL per well of a 1:30 dilution after acidification treatment of serum and plasma samples; cell/tissue culture samples may require dilution prior to acidification treatment (see protocol for details).
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or alternatively, 2 hours at room temperature (20-25°C).
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Cardiovascular Disease, Chronic Inflammatory Disease, Immune Disorders, Inflammatory Disease, Rheumatoid Arthritis

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Statements

H290,H314,H317,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

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Samantha M Logan et al.
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We aimed to explore the immunological characteristics and cytokine profile of the initial stage of type 1 diabetes. In total, 123 age- and sex-matched subjects with newly diagnosed classic type 1 diabetes mellitus (T1ADM), fulminant type 1 diabetes mellitus (FT1DM)
Maria Björmsjö et al.
Journal of pediatric gastroenterology and nutrition, 74(3), e65-e72 (2021-12-16)
Compared to formula-fed infants, breastfed infants have a lower risk of infections. Two possible reasons for this are the presence of the anti-infective and anti-inflammatory protein lactoferrin and the lower level of iron in breast milk. We explored how adding
Decha Pinkaew et al.
Communications biology, 5(1), 157-157 (2022-02-25)
Fortilin is a 172-amino acid multifunctional protein present in both intra- and extracellular spaces. Although fortilin binds and regulates various cellular proteins, the biological role of extracellular fortilin remains unknown. Here we report that fortilin specifically interacts with TGF-β1 and
Inmaculada Parrilla et al.
Animals : an open access journal from MDPI, 10(11) (2020-11-08)
In the context of porcine embryo transfer (ET) technology, understanding the tightly regulated local uterine immune environment is crucial to achieve an adequate interaction between the transferred embryos and the receiving endometrium. However, information is limited on the uterine immune
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