ST1705

Anti-TOMM20 Mouse mAb (4F3)

liquid, clone 4F3, Calbiochem^®

• Synonym(s): Anti-Translocase of Outer Mitochondrial Membrane 20
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4F3, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human, rat, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TOMM20(9804)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein.

Recognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue.

This Anti-TOMM20 Mouse mAb (4F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM20.

Immunogen

Full-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein

Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
HeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable