SCT083

BioTracker^™ MemBright 488 Live Cell Dye

• Synonym(s): Live cell imaging probe, Live cell membrane probe, green
• UNSPSC Code: 41106514
• NACRES: NA.47

Properties

• mol wt: observed mol wt 1218.5 kDa
• Quality Level: 100
• packaging: vial of 50 μL
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell based assay: suitable; flow cytometry: suitable
• detection method: fluorometric
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The BioTracker^™ Membright-488 live cell dye is a “turn-on” fluorescence probe emitting in the far red spectrum for the imaging of plasma membranes of live cells.

Specific plasma membrane markers are essential for delineating the cell. Membright dyes exploit a unique fluorogenic mechanism, existing in a self-quenched nanoparticle state. Fluorescence is turned on upon contact with and propagation within the plasma membrane, producing bright, background-free signal. The Membright live cell membrane dyes are compatible with two-photon imaging, superresolution imaging, and fixed cell preparations. The high photostability and lack of cytotoxicity of Membright dyes make them suitable for long-term live cell imaging. The suite of superior characteristics of Membright plasma membrane dyes permit use in a wide spectrum of fluorescence microscopy applications and flow cytometry.

Spectral Properties
Fluorescence images obtained by λex = 488 nm and emission range of 500-550 nm.

Reference

Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614

Application

Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.

Features and Benefits

Green fluorescent probe for efficient, selective labeling of the plasma membrane in live cells. Suitable for superresolution microscopy.

Physical form

Green solution

Storage and Stability

Store long term at -20°C, protected from light.

Other Notes

Live cell fluorescent imaging, flow cytometry

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup