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SCGP00525

Millipore

Millipore® Steriflip® Vacuum Tube Top Filter

pore size 0.22 μm, polyethersulfone membrane, funnel capacity 50 mL, pack of 25 ea

Synonym(s):

Steriflip-GP Sterile Centrifuge Tube Top Filter Unit, tube top sterile filter unit, tube top vacuum filter, tube top vacuum sterile filtering unit

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About This Item

UNSPSC Code:
41104914
eCl@ss:
32031602
NACRES:
NB.22

material

acrylic housing
polyethersulfone membrane
polypropylene

Quality Level

sterility

sterile

product line

Steriflip®

feature

holdup volume 0.6 mL
hydrophilic

packaging

pack of 25 ea

parameter

45 °C max. temp.

technique(s)

sterile filtration: suitable

H

138 mm

diam.

6.4 cm

filter diam.

40 mm

filtration area

7 cm2

funnel capacity

50 mL

matrix

Express PLUS

pore size

0.22 μm pore size

fitting

double lead thread inlet (with vacuum port)
double lead thread outlet for 50 mL centrifuge tube

shipped in

ambient

General description

Compatible with 50 mL centrifuge tubes, Steriflip® filters eliminate the liquid transfer step by integrating directly into the sample tube. To filter, attach the unit to the 50 mL centrifuge tube containing your sample, flip, and apply vacuum. The filtrate collects directly into the attached 50 mL tube.

This Steriflip® 0.22 μm pore size polyethersulfone (PES) membrane filter unit combines a 50 mL centrifuge tube with a cap for processing and storage.

Application

For Research Use Only

Steriflip® filters are designed to be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.
  • Tissue culture media +/- additives
  • Buffers
  • Biological solutions

Features and Benefits

  • Eliminates messy sample transfer via a simple vacuum-assisted, closed system
  • Can isolate cells from cellular debris using any of the various pore-sized nylon net filters
  • Ideal for sterilizing small batches of culture media
  • Attaches to any standard 50 mL centrifuge tube, eliminating unnecessary materials, time and risk of spillage

Legal Information

EXPRESS PLUS is a trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
Steriflip is a registered trademark of Merck KGaA, Darmstadt, Germany

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Protocols

Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.

This page shows an example protocol of an ELISpot assay for quantifying the cells producing interferon-gamma in response to antigen or non-specific activation using phytohemagglutinin.

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