MABC547

Anti-Poly ADP-ribose Antibody, clone 10H

clone 10H, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 10H, monoclonal
• species reactivity: human
• technique(s): immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG3κ
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PARP1(142)

Description

General description

Poly(ADP-ribose) polymerase is an abundant nuclear enzyme that catalyses the synthesis of poly(ADP-ribose) from nicotinamide adenine dinucleotide (NAD+). Poly(ADP-ribose) has an N-terminal DNA-binding domain containing two zinc-fingers, which is linked to the C-terminal NAD+-binding domain by a short region containing several glutamic acid residues that are sites of auto-poly (ADP-ribosyl) ation. Production of poly(ADP-ribose) within the cell is initiated by agents that generate DNA strand interruptions. The branched homopolymer chains may reach a length of 200–300 residues but are rapidly degraded after synthesis. The function of poly(ADP-ribose) synthesis is not clear, although it seems to be required for DNA repair.

Immunogen

Corresponding to human Poly ADP-ribose chain.

Application

Detect PARP using this mouse monoclonal antibody, Anti-Poly ADP-ribose Antibody, clone 10H validated for use in western blotting, IP & Immunofluorescence.

Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to immunoprecipitate Poly ADP-ribose in cultured supernatents from mouse erythrocytes coated with Poly ADP-ribose (Kawamitsu, H., et al. (1984). American Chemical Society. 3771-3777).
Immunofluorescence Analysis: A representative lot was used by an an independent laboratory to detect Poly (ADP-ribose) synthesis on transfected CV-1 cells via indirect immunofluorescence (J.H. Kupper, et al. J. Biol. Chem. (1990) 265:18721).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in untreated and UV treated HeLa cells.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected UV treated HeLa cells. Little or no signal observed in untreated HeLa cells.

Target description

Observed molecular weight is the result of poly(ADP-ribose) polymer on protein receptors (such as histones and transcription factors).

Linkage

Replaces: MAB3192

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable