MAB5434-C

Anti-Oct-1 Antibody, clone YL15, Ascites Free

clone YL15, from mouse

• Synonym(s): POU domain, class 2, transcription factor 1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1, OTF-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: YL15, monoclonal
• species reactivity: human, mouse
• technique(s): ChIP: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001185712
• UniProt accession no.: P14859
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... POU2F1(5451) ; mouse ... Pou2F1(18986)

Description

General description

POU domain, class 2, transcription factor 1 (UniProt P14859; also known as NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1, OTF-1) is encoded by the POU2F1 (also known as OCT1, OTF1) gene (Gene ID 5451) in human. Oct-1 belongs to the family of octamer transcription factors characterized by their binding to the ATTTGCAT octamer sequence via their POU domain (a.a. 280-354 of human Oct-1). Oct1 is widely expressed in adult tissues, however, Oct-1 knockdown has little impact on cell growth and viability in culture. Oct-1 controls multiple stem cell phenotypes in normal and tumor cells. Oct-1 promotes resistance to genotoxic and oxidative stresses in stem cells. Loss of Oct-1 antagonizes oncogenic transformation in vitro and tumorigenicity in vivo.

Specificity

Clone YL15 targets the POU-domain linker region (Herr, W., and Cleary, M.A. (1995). Genes Dev. 9(14):1679-1693; Lai, J.S., and Herr, W. (1992). Proc. Natl. Acad. Sci. U. S. A. 89(15):6958-6962) present in all six spliced isoforms of human Oct-1 (UniProt P14859). Clone YL15 does not react with Oct-2, Oct-4 or Oct-6 (Maddox, J., et al. (2012). PLoS Genet. 8(11):e1003048).

Immunogen

Epitope: POU-domain linker region.

Recombinant full-length human Oct-1.

Application

Anti-Oct-1 Antibody, clone YL15, Cat. No. MAB5434-C, is a mouse monoclonal antibody that detects octamer-binding protein 1 (Oct-1) and has been tested for use in immunocytochemistry, immunohistchemistry (Parffin), Immunoflouroesencece, Western blotting, and Chromatin Immunorecipitation.

Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot detected Oct-1 cellular localization in human A431 and HeLa cells by fluorescent immunocytochemistry.
Western Blotting Analysis: A representative lot detected Oct-1, but not Oct-2, Oct-4, or Oct-6 in various human cell lysates and nuclear extracts (Maddox, J., et al. (2012). PLoS Genet. 8(11):e1003048).
Western Blotting Analysis: A representative lot detected Oct-1 in Burkitt lymphoma cells (BJAB and Namalwa), as well as a panel of six primary effusion lymphoma (PEL) cells (Di Bartolo, D.L., et al. (2009). J. Virol. 83(9):4308-4315).
Immunofluorescence Analysis: A representative lot detected Oct-1 immunoreactivity in paraformaldehyde-fixed human and mouse frozen colon tissue sections by fluorescent immunohistochemistry (Maddox, J., et al. (2012). PLoS Genet. 8(11):e1003048).
Immunohistochemistry Analysis: A representative lot detected Oct-1 immunoreactivity in formalin-fixed, paraffin-embedded human colon tissue sections (Maddox, J., et al. (2012). PLoS Genet. 8(11):e1003048).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Oct-1 occupancy at the ORF50 promoter, using stable Oct-2-inducible primary effusion lymphoma (PEL) cell line BC1/Tet-on/Oct-2. The association decreased upon induction of Oct-2 expression and enhanced upon ORF50 transfection (Di Bartolo, D.L., et al. (2009). J. Virol. 83(9):4308-4315).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Oct-1 occupancy at the U6 and U1 promoter regions in exponentially dividing HeLa cells (Zhao, X., et al. (2001). Mol. Cell. 7(3):539-549).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of radiolabeled OCT1 consensus oligonucleotide-bound complex in the nuclear extracts from Burkitt lymphoma cells BJAB and Namalwa (Di Bartolo, D.L., et al. (2009). J. Virol. 83(9):4308-4315).
Immunocytochemistry Analysis: A representative lot detected Oct-1 cellular localization in human diploid fibroblast MRC-5-derived HuS-L12 cells at various population-doubling levels (PDLs), as well as in the corresponding immortalized IML12–4 cells by fluorescent immunocytochemistry (Imai, S., et al. (1997). Mol. Biol. Cell. 8(12):2407-2419).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Oct-1 from HEK293 cell lysates (Lai, J.S., and Herr, W. (1992). Proc. Natl. Acad. Sci. U. S. A. 89(15):6958-6962).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Oct-1 in 10 µg of HeLa cell lysate.

Target description

~96 kDa observed.

Linkage

Replaces: MAB5434

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable