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AB5042P

Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

Chemicon®, from rabbit

Synonym(s):

ChAT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

NCBI accession no.

NM_020549.3
NM_020984.2
NM_020985.2
NM_020986.2

UniProt accession no.

P28329

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Chat(290567)

Specificity

Choline acetyltranferase. The antibody will stain cholinergic neurons in the rat central and peripheral nervous systems.

Immunogen

A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, mouse primate and human ChAT.

Application

Detect Choline Acetyltransferase using this Anti-Choline Acetyltransferase Antibody validated for use in ELISA, IH.
Immunohistochemistry: 1:2,000-1:4,000 in rat basal forebrain.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Lyophilized. Contains no preservative. Reconstitute with 20 μL of sterile distilled water. Centrifure to remove any insoluble material.

Storage and Stability

Maintain lyophilized material dry at -70°C for up to 12 months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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I Sukhotinsky, V Zalkind, J Lu, D A Hopkins, C B Saper, M Devor, I Sukhotinsky, V Zalkind et al.
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Hypoglycemia activates arousal-related neurons and increases wake time in adult rats.
Nancy C Tkacs,Yanhua Pan,Gagan Sawhney,Graziella L Mann,Adrian R Morrison
Physiology & Behavior null
Antibodies raised against synthetic peptides react with choline acetyltransferase in various immunoassays and in immunohistochemistry.
Benecke, S, et al.
Journal of Neurochemistry, 61, 804-811 (1993)
Human embryonic germ cell derivatives facilitate motor recovery of rats with diffuse motor neuron injury.
Douglas A Kerr, Jeronia Llado, Michael J Shamblott, Nicholas J Maragakis, David N Irani et al.
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