AB1832P
Anti-Interleukin-1β Antibody
Chemicon®, from rabbit
Synonym(s):
IL-1beta
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
neutralization: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
rat ... Il1B(24494)
Specificity
Reacts with rat Interleukin-1beta (IL-1beta).
Immunogen
Highly pure (>98%) recombinant rat IL-1beta.
Application
Anti-Interleukin-1β Antibody is an antibody against Interleukin-1β for use in ELISA, WB, NEUT.
ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of
0.2-0.4 ng/well when rat IL-1(is used to coat the plate.
Western blot: 0.1-0.2 μg/mL, this dilution should offer detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Neutralization: to yield ½ maximal inhibition (ND50) of the biological activity of rat IL-1beta (0.2 ng/mL), a concentration of 0.09-0.14 μg/mL of antibody is required.
Final working dilutions must be determined by end user.
0.2-0.4 ng/well when rat IL-1(is used to coat the plate.
Western blot: 0.1-0.2 μg/mL, this dilution should offer detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Neutralization: to yield ½ maximal inhibition (ND50) of the biological activity of rat IL-1beta (0.2 ng/mL), a concentration of 0.09-0.14 μg/mL of antibody is required.
Final working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
Linkage
Replaces: 04-462; MABF22
Physical form
Affinity purified Immunoglobulin. Lyophilized from 0.5X PBS pH 7.4, no preservatives. Reconstitute in sterile distilled water.
Storage and Stability
Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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