553211

Rapamycin

≥95% (HPLC), solution, mTOR inhibitor, Calbiochem

• Synonym(s): InSolution Rapamycin, mTOR Inhibitor I
• Empirical Formula (Hill Notation): C₅₁H₇₉NO₁₃
• Molecular Weight: 914.17
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: Rapamycin, Ready Made Solution, 2.5 mg/mL in DMSO (2.74 mM), from Streptomyces hygroscopicus
• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: desiccated (hygroscopic); protect from light
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Selectively inhibits the phosphorylation and activation of p70 S6 kinase (IC₅₀ = 50 pM). Prevents the translational activation of IGF-II. Also prevents resting T-cells from entering the cell cycle, but does not directly arrest cell cycle progression. Shown to inhibit later signaling events, such as p110Rb phosphorylation, p34cdc2 kinase activation, and cyclin A synthesis. Exhibits strong binding to FK-506 binding proteins. Also reported to induce apoptosis in a murine B-cell line, to inhibit lymphokine-induced cell proliferation at the G₁ phase, and to irreversibly arrest Saccharomyces cerevisiae cells in the G₁ phase.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Mammalian target of rapamycin (mTOR)

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50 pM inhibiting the mammalian target of rapamycin (mTOR) and blocking the subsequent activation of p70 S6 kinase

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

2.5 mg/mL solution in DMSO (2.74 mM)

Reconstitution

Following initial use, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flash Point(C): 87 °C - closed cup - (Dimethylsulfoxide)