362180

N-Glycosidase A, Almond

Native N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine. Note: 1 mU = 1 milliunit.

• Synonym(s): N-Glycosidase A, Almond
• CAS Number: 83534-39-8
• Enzyme Commission number: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD00131222
• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• form: liquid
• specific activity: ≥500 mU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• foreign activity: α- and β-galactosidase, β-glucosidase ≤0.1%; proteases, none detected
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Native N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine.

Native N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine. Has an optimal pH of 4.0-6.0.

Native N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine. Note: 1 mU = 1 milliunit.

Note: 1 mU = 1 milliunit.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol hybrid and high-mannose oligosaccharides from ovalbumin glycopeptide per min at 37°C, pH 5.0.

Physical form

In 50 mM citrate/phosphate buffer, 50% glycerol, pH 5.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
Takahashi, N., and Nishibe, H. 1978. J. Biochem. 84, 1467.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2