1.52016

Chromolith^® RP-18 endcapped

L × I.D. 100 mm x 10 mm SemiPrep column

• Synonym(s): Chromolith^® SemiPrep RP-18 endcapped 100-10 column
• UNSPSC Code: 41115709

Properties

• material: PEEK column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: Chromolith^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 18% Carbon loading
• parameter: 150 bar pressure; 45 °C max. temp.
• technique(s): HPLC: suitable (Semi Prep HPLC); LC/MS: suitable
• column L × I.D.: 10 cm × 10 mm
• matrix: monolithic silica
• matrix active group: C18 bonding phase
• pore size: 13 nm mesoporosity; 2 μm macroporosity
• operating pH range: 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

Chromolith^® SemiPrep RP-18 endcapped HPLC columns are ideally suited for direct scale-up from analytical to semiprep. This is because they offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles. Chromolith^® SemiPrep 10 mm i.d. columns combine high separation speed with excellent performance. This makes them the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d.). They have the same bimodal porous silica rod structure as Chromolith^® analytical columns with an internal diameter of 4.6 mm. Their macropores are 2 μm in diameter and the mesopores are 13 nm. This combination dramatically reduces separation time while increasing efficiency.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes

The recommended Guard-columns are:
Chromolith^® SemiPrep RP-18 endcapped 10-10 guard cartridges (3 pieces) [1.52036] with the Chromolith^® Guard Cartridge Holder 10 mm x 10 mm [1.52037]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

Analysis Note

Carbon content: 18.0 - 22.0 %
Pressure: ≤ 30 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 4.3 - 5.0
Tailing (USP): 0.90 - 1.45 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany