06-255-I

Anti-JAK2 Antibody

from rabbit

• Synonym(s): Tyrosine-protein kinase JAK2, JAK2, Janus kinase 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_001041642
• UniProt accession no.: Q62120
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... JAK2(3717) ; mouse ... Jak2(16452) ; rat ... Jak2(24514)

Description

General description

Tyrosine-protein kinase JAK2 (EC 2.7.10.2; UniProt Q62120; also known as JAK-2, Janus kinase 2) is encoded by the Jak2 (also known as Fd17) gene (Gene ID 16452) in murine species. JAK2 belongs to the family of non-receptor Janus tyrosine kinases that regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines cause the recruitment of Stat transcription factors to cytokine receptor. Subsequent phosphorylation by receptor-associated JAK2 causes Stat dimerization, nuclear translocation, and Stat-mediated target genes transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering JAK/Stat signaling to reduce immune cells cytokine responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor.

Immunogen

Epitope: Residues 758-776

KLH-conjugated linear peptide corresponding to residues 758-776 of mouse JAK2.

Application

Research Category
Signaling

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected JAK2 in 10 µg of RAW264.7 cell lysate.

Target description

~130 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable