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Key Documents

SAB2702214

Sigma-Aldrich

Monoclonal Anti-RFP antibody produced in mouse

clone GT1433, affinity isolated antibody

Synonym(s):

Mouse Anti-Ref Fluorescent protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT1433, monoclonal

form

buffered aqueous solution

mol wt

27kDa

concentration

0.98mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 5000-20000

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Red fluorescent protein (RFP) is obtained from the Discosoma coral. It can be used as a fluorescent probe for optical microscopy and acts as a biological marker for biomolecule imaging. RFPs exhibit fluorescence emission beyond 57 0nm. They have lower light-scattering, low photo-toxicity and less autofluorescence interference of cells. RFPs are extremely useful for whole body and deep tissue imaging.

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP.

Application

Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Monoclonal Anti-RFP antibody produced in mouse has been used in chromatin immunoprecipitation (ChIP) assay.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast*
Pradeep Kumar Yadav and Ram Rajasekharan
The Journal of Biological Chemistry (2016)
DNA mimics of red fluorescent proteins (RFP) based on G-quadruplex-confined synthetic RFP chromophores
Nucleic Acids Research, 45(18) (2017)
A monomeric red fluorescent protein
Robert E. Campbell
Proceedings of the National Academy of Sciences of the USA (2002)
Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry
Kiryl D
Methods in Cell Biology (2011)
Pradeep Kumar Yadav et al.
The Journal of biological chemistry, 291(35), 18562-18581 (2016-07-13)
The DDHD domain-containing proteins, which belong to the intracellular phospholipase A1 (iPLA1) family, have been predicted to be involved in phospholipid metabolism, lipid trafficking, membrane turnover, and signaling. Defective cardiolipin (CL), phosphatidylethanolamine, and phosphatidylglycerol remodeling cause Barth syndrome and mitochondrial
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