B8429

Anti-Bax antibody, Mouse monoclonal

clone 6A7, purified from hybridoma cell culture

• Synonym(s): Anti-BCL2L4
• MDL number: MFCD00282585
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6A7, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 21 kDa (and possibly a dimer of 42 kDa)
• species reactivity: mouse, rat, human
• concentration: 2 mg/mL
• technique(s): immunoprecipitation (IP): suitable; microarray: suitable; western blot: 10-60 μg/mL using extract of human breast adenocarcinoma MCF-7 cells, activated with dexamethasone
• isotype: IgG1
• UniProt accession no.: Q07812
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BAX(581) ; mouse ... Bax(12028) ; rat ... Bax(24887)

Description

General description

Bax is a 21kD integral organelle membrane protein that belongs to the Bcl-2 family and is expressed mainly in the mitochondria. The formation of bax homodimer accelerates apoptotic death whereas its heterodimerization with Bcl-2 or Bcl-xL can block apoptosis. Monoclonal anti-Bax antibody can be used to study the intracellular redistribution of Bax protein upon induction of apoptosis and its unique subcellular localization. This product can also be used in immunoblot analysis to estimate variations in the expression of specific proteins involved in apoptosis signalling. Mouse anti-Bax antibody reacts specifically with the epitope present within the amino acids 12-24 shared by bax from rat, mouse and human.

The B-cell lymphoma 2 (BCL2) associated X, apoptosis regulator (BAX) is mapped to human chromosome 19q13.33.

Immunogen

synthetic peptide corresponding to amino acids 12-24 of the human Bax sequence, conjugated to KLH.

Application

Anti-Bax antibody, Mouse monoclonal has been used in the western blotting detection in human retinal pigment epithelium cell lines, glioma, and breast cancer cell lines.

Monoclonal anti-Bax antibody can be used in immunocytochemistry (diluted 1: 1500) and western blot. It is also useful in microarray and immunoprecipitation.

Biochem/physiol Actions

Mutations in the B-cell lymphoma 2 (BCL2) associated X, apoptosis regulator (BAX) gene is implicated in colorectal cancer. BAX is associated with ovarian follicular dysfunction.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable